A computational analysis demonstrating vitamin E nicotinate promoting anti-inflammatory signals

Abstract Vitamin E nicotinate is an ester of tocopherol (vitamin E) and niacin B3). Niacin linked to the hydroxyl group active vitamin E, blocking free radical scavenging properties. While this can be chemically synthesized, whether formed from in biological system unclear. Metabolomics analysis performed our laboratory demonstrated that level 30-fold lower rats suffering inflammatory state. The rat diet contained acetate separately (but not form nicotinate), only other forms was decreased, suggesting system. To test if functions independently simply serving as a source we also metabolomics compare effects on cultured cells those + added separately. Results revealed treated with for 10 min had significantly higher levels molecules anti-inflammatory properties including anandamide, virodhamine, palmitamide, oleamide, oleoylethanolamide. niacin) phosphorylated activated extracellular signal-regulated kinase (ERK), which serves signal. These results suggest new function molecule through non-direct effects. Supported by grants NIH (R21 AG73919, R03 AG71596)